Lectures
Theoretical-Practical classes
Best practices:
- Keep all files and software like ApE in one folder
- Use the Editpad Online text editor (or similar) to collect responses to the questions
- Each document contain around 5-10 questions. The student should to keep a text file with answers in this format:
| # | Title |
|---|---|
| TP01 | Calculating checksums using seguid calculator |
| TP02 | Visualizing linear and circular DNA with ApE |
| TP03 | In-silico sub cloning using ApE |
| TP04 | Manual PCR simulation |
| TP05 | Primer design and melting temperature calculations for PCR primers |
| TP06 | How to download sequences from Genbank |
| TP07 | Genbank and the Genbank flat file format |
| TP08 | Saccharomyces Genome Database |
| TP09 | Cloning by HR and Gibson assembly |
| TP10 | Searching databases using BLAST |
| TP11 | Fungal protein BLAST |
| TP12 | Restriction mapping |
| TP13 | Agarose gel electrophoresis |
| TP14 | how to take the bioinformatics test on your own computer |
| TP15 | Sanger sequencing 🚧 |
| TP16 | Sequence comparison using dotlet |
| TP17 | Picking primers for cloning and sequencing with Primer3Plus |
| TP18 | Reproducing a cloning strategy from literature |
| TP19 | Picking primers with ApE |
| TP20 | Assembly of pTAx vectors |
| TP21 | Automatic PCR product simulation using WebPCR |
| TP22 | Assembly of a yeast gene deletion cassette 🚧 |
| TP23 | Fusion protein design |
| TP24 | Small scale assembly of sequence reads |
| TP25 | GoldenGateCloning |
| TP26 | Simply Cloning |
| TP28 | Metabolic yield calculations 🚧 |
| TP29 | 🚧 |
| TP30 | 🚧 |
| TP31 | Primer design and cloning genetic elements in pYPKa 🚧 |
| TP32 | 🚧 |
| TP33 | DNA calculations |
| TP34 | Assembly of a TU using YPK |
| TP35 | Chemical Thermodynamics |
| TP36 | Metabolism |
| TP37 | Metabolism II |