A series of ten YouTube videos by Andriy Nemirov describing a sub cloning experiment from planning to verification of the final construct. total run time is 65 minutes.
Simply Cloning - Chapter 1 - Planning 12:27
Simply Cloning - Chapter 2 - PCR 06:39
Simply Cloning - Chapter 3 - Vector Restriction Digest 02:52
Simply Cloning - Chapter 4 - Gel Purification 11:47
Simply Cloning - Chapter 5 - Insert Restriction Digest 02:55
Simply Cloning - Chapter 6 - Ligation 06:10
Simply Cloning - Chapter 7 - Transformation 08:02
Simply Cloning - Chapter 8 - Plasmid Miniprep 06:05
Simply Cloning - Chapter 9 - Clone Verification 05:20
Simply Cloning - Chapter 10 - Clone Library 02:48
Questions
Question 1: What is the cdseguid of the pSAT6-Bar vector?
Question 2: Why does he run the gel in Chapter 4?