Synthetic Dextrose Minimal Medium (SD) also called Synthetic Minimal Glucose Medium or Synthetic Defined (SD). SD is a synthetic minimal medium containing salts, trace elements, vitamins, a nitrogen source, and glucose.
- 1.7 g/L Yeast Nitrogen Base (YNB) WITHOUT AMINOACIDS AND Ammonium sulfate
- 20 g/L glucose
- Optional: 20 g/L Agar for solid medium
Mix the dry components in a bottle and add water. All components can be autoclaved together.
Plain YNB has Histidine Methionine and Tryptophan added. This may or not interfere with the experiment.
IMPORTANT
Use the correct Yeast Nitrogen Base!
Alternatively, the medium can be made by combining sterile stock solutions (1L):
- 100 mL 10x YNB
- 40 mL 50% Glucose
- Water and optionally agar to 1L
Adding amino acids
Sometimes yeast strains need amino acid supplementation of growth. Add amino acids amino acid drop out mix.