YNB

Protocol for making x 10 times concentrated YNB (Yeast Nitrogen Base) solution

The final concentration of YNB in media depends on the type of YNB

Component	C (g/L)	Ammonium sulfate	Amino acids
YNB w/o amino acids w ammonium sulfate	6.7	✅	✅
YNB	6.7	✅	❌
YNB w/o ammonium sulfate	1.7	❌	❌

YNB has:

• L-Histidine HCl
• DL-Methionine
• DL-Tryptophan

YNB w ammonium sulfate has 5 g/L ammonium sulfate.

10 x YNB w ammonium sulfate w amino acids

Dissolve 13.4 g YNB w (NH4)2SO4 in 200 mL water. Sterilize by autoclaving.

10 x YNB w/o ammonium sulfate

Dissolve 13.4 g YNB w/o (NH4)2SO4 in 200 mL water. Sterilize by autoclaving.

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Composition

• oww oww

Yeast_Nitrogen_Base_(YNB),_per_liter

6.7 g/L

L-His
LD Me
LD Trp

YNB has HMT Histidine Methionine Tryptophan

Yeast Nitrogen Base (YNB), per liter

Ammonium sulfate 5 g
Potassium phosphate monobasic 1 g
Magnesium sulfate 500 mg
Sodium chloride 100 mg
Calcium chloride 100 mg

• Inositol 2000 μg
• Calcium pantothenate 400 μg
• niacin 400 μg
• Pyridoxine hydrochloride 400 μg
• Thiamine hydrochloride 400 μg
• p-aminobenzoic acid 200 μg
• [[|Riboflavin]] 200 μg
• [[|Folic acid]] 2 μg
• Biotin 2 μg

Boric acid 500 μg
Copper sulfate 40 μg
Potassium iodide 100 μg
Ferric chloride 200 μg
Manganese sulfate 400 μg
Sodium molybdate 200 μg
Zinc sulfate 400 μg

Final pH 4.5 before addition of Ammonium sulfate (5 g/liter)
Final pH 5.4 after addition of Ammonium sulfate (5 g/liter)

Difco 0919-15 Yeast nitrogen base w/o amino acids 100g
Difco 0335-15 Yeast nitrogen base w/o amino acids and ammonium sulfate 100g