- 2x PCR mastermix
- Agar 40 g/L (2x)
- 40% w/v Glucose stock solution for media
- 40% w/v Sucrose stock solution for media
- 25% w/v NaCl stock solution for media
- 3.5M KCl stock solution.
- 2.5M MgCl2 stock solution.
- 2M MgSO4 stock solution.
- 0.5 M EDTA stock solution, pH 8
- 1.0 M TrisHCl, pH 8
- 1M IPTG stock solution
- 200 mg/mL Ampicillin solution (x1000)
- 200 mg/mL Geneticin solution (x1000)
- 12.5 mg/mL Tetracycline solution (x1000)
- 10 mM dNTPs solution
- 5x Ligase buffer
- DNA DNA loading-buffer with FICOLL and two dyes.
- PBS
- antibiotics