The thirty three sequences below were designed with the R2oDNA designer tool to be biologically inert.
>s1 ❶
AATCCAATCAGCGTAAGGTGTAGACTTTCT
>s2 ❷
ATCGTATCTGCTGCGTAAATAGTAGTCAAC
>s3 ❸
TAAAATCTCGTAAAGGAACTGTCTGCTCTG
>s4 ❹
AACTGTAAAATCAGGTATCTCGTAGTCCGT
>s5 ❺
GAAAAGCGTTTACCTCGGAACTCTATTGTA
>6
TTTACCTGTGGATTGATAGGAATACACCCA
>7
TTGAGATAGCAATAAATCCTGGTTCCGACT
>8
TAAACACAGTAACGGATTGCTTTCGTGCTA
>9
CTCGTGAATCTGGGAAATCCTTATCGTAAA
>10
AGTTATTACGAGTATTCAACCTGACGGCTA
>11
TGGTAACTGCGAACCTACTGTAAGATTCTA
>12
GTAGGTATTGAACCCGAAAATCGTATCTTC
>13
ATAATCTATCTGTAGGACCTGGTAGTCACA
>14
AAAGTATTCAGCGACTATTGGCTTCTACGA
>15
GTAGTTTCTTACAATAACGCCGATACAGGT
>16
AGGTAAAATCGGTCCCTTATCTACTGTGAA
>17
AAGATTGGTATCCCTCACGACTGTAGTAAT
>18
GTCGTAAATACAATCTCGGAATAGGCTCTT
>19
TGTAAAGTGGTAATCTTCAATCCAGAGCCT
>20
AATCTCTTCCTGGTGACACTGAATAAGGTA
>21
TGATAGCGAGTTGCCTCCAATAAAATCGTT
>22
CTTTATTTCGTAGACGCTATCAGAGACAGA
>23
ATAAGTGTCTCCCGTTGATTCTGAAAAGCA
>24
GTGAGTAGACTTATTGTTTACCCAACCGAA
>25
GAGAGTATTTACAACGCAGTCTTCGTCAAT
>26
AGGTGCTCTTATTATCGGACAGTCAATCAA
>27
GAAACTTATCGTTCAATAGGGTTCCCGTAA
>28
TCAGAAGTCTCCAATAAGGTTGTGATACTC
>29
AGAGGTTTTATTACAACAGGTCCACTTCAG
>30
ATCTGCGGTATCTCAGTAATAAGAATCGCT
>31
TAGGGCACTCTACAATAATCTTCTGGTAAG
>32
ATTATTGGGTGAACCACACGAGTAACCTTT
>33
TGGGTTCAACAATAAACTTTCGCAGGTATC
The text below was generated from the software tool:
ProjectName
JobID r2o_6389398447409610491
Email bjorn_johansson@bio.uminho.pt
Seqnum 6
Format NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
ReverseMode false
Evalue 1
Genomes saccharomyces_cerevisiae_genome eschericichia_coli_k12_dh10b eschericichia_coli_k12_mg1655 eschericichia_coli_k12_w3110 bacillus_subtilis_168 igem_all_parts_082013
gc 0.4
MinInterEnergy -9.0
MinIntraEnergy -4.0
FoldTemp 50.0
MaxSWScore 90.0
MaxSubMatch 10
ForbSeq TTGACA E.coli sig70 -35 site
ForbSeq TATAAT E.coli sig70 -10 site
ForbSeq TTGNNNNNNNNNNNNNNNNNNNNTATNNT E.coli sig70 promoter weak consensus
ForbSeq TGGCACGNNNNTTGC E.coli sig54 promoter consensus
ForbSeq TCNCCCTTGAANNNNNNNNNNNNNNNNNCCCCATTTA E.coli sig32 promoter consensus
ForbSeq GAACTNNNNNNNNNNNNNNNNGTCNNA E.coli sig24 promoter consensus
ForbSeq AAAGA RBS
ForbSeq AGGAGG Shine-Dalgarno sequence or 2xArg bad codon
ForbSeq ATG translation initiation sites
ForbSeq TTATNCACA DnaA binding sites
ForbSeq TGTGANNNNNNTCACANT CAP binding sites
ForbSeq CTAG IS5 insertion site
ForbSeq CTAA IS5 insertion site
ForbSeq CAAA IS5 insertion site
ForbSeq CAAG IS5 insertion site
ForbSeq NGCTNAGCN IS10 insertion site
ForbSeq GGGNNNNNCCC IS231 insertion site
ForbSeq G{3,}[ATGC]{1,7}G{3,}[ATGC]{1,7}G{3,}[ATGC]{1,7}G{3,} G-quadruplex
ForbSeq GGGG G-quadruplex
ForbSeq GAATTC EcoRI
ForbSeq ACTAGT SpeI
ForbSeq TCTAGA XbaI
ForbSeq CTGCAG PstI
ForbSeq GGTCTC BsaI
ForbSeq AAAAA run
ForbSeq TTTTT run
ForbSeq GGGGG run
ForbSeq CCCCC run
ForbSeq ATATAT run
ForbSeq ACACAC run
ForbSeq AGAGAG run
ForbSeq TATATA run
ForbSeq TCTCTC run
ForbSeq TGTGTG run
ForbSeq CACACA run
ForbSeq CTCTCT run
ForbSeq CGCGCG run
ForbSeq GAGAGA run
ForbSeq GTGTGT run
ForbSeq GCGCGC run
ClusterID 4206134
sequencesStep1File.fa: 60 #after initial sequence generation
sequencesStep1File_pf_self.fa: 60 #after folding and self-annealing energy filter
sequencesStep2File.fa: 43 #after BLAST elimination
sequencesFinalFile.fa: 33 #final orthogonal sequence count after network elimination