ZraI
5'...GACGTC...3'
3'...CTGCAG...5'
5'...GAC GTC...3'
3'...CTG CAG...5'
AjiI or BmgBI
5'...CACGTC...3'
3'...GTGCAG...5'
5'...CAC GTC...3'
3'...GTG CAG...5'
EcoRV or Eco32I
5'...GATATC...3'
3'...CTATAG...5'
5'...GAT ATC...3'
3'...CTA TAG...5'