ZraI 5'...GACGTC...3' 3'...CTGCAG...5' 5'...GAC GTC...3' 3'...CTG CAG...5' AjiI or BmgBI 5'...CACGTC...3' 3'...GTGCAG...5' 5'...CAC GTC...3' 3'...GTG CAG...5' EcoRV or Eco32I 5'...GATATC...3' 3'...CTATAG...5' 5'...GAT ATC...3' 3'...CTA TAG...5'