TAE buffer is commonly prepared as a 50× stock solution for laboratory use.
This stock solution can be diluted 49:1 with water to make a 1× working solution.
| Name | Per 1 mole | 50x solution | 50× | 1× solution |
|---|---|---|---|---|
| Tris base | 121.1 g/L | 2 M | 242.2 g/L | 40 mM |
| acetic acid | 57.1 ml/L | 1 M | 57.1 ml/L | 20 mM |
| EDTA disodium salt dihydrate | 372.24 g/L | 50 mM | 18.612 g/L | 1 mM |
| IMPORTANT! Use Tris base, not Tris-HCl. |