Summary: T6 - How to clone a new gene
What is this TP about?
This TP focuses on strategies to isolate one specific gene from a complex genome.
Approaches
- Genomic libraries
- cDNA libraries
- PCR-based cloning
- Hybridisation-based screening
Screening Methods
- Colony hybridisation
- PCR screening
- Functional complementation
Key Take-Home Message
Isolating a gene is a problem of strategy and selection, not chance.
Literature
Brown T. Gene Cloning and DNA Analysis - An Introduction 8ed (2020)
- Chapter 08 How to Obtain a Clone of a Specific Gene video