Summary: T5 - Purification of DNA from Living Cells
What is this TP about?
This TP explains how to isolate DNA from cells without degradation and in a form suitable for downstream applications.
Main Steps
- Cell lysis
- Removal of proteins and RNA
- DNA precipitation and purification
Types of DNA Isolated
- Genomic DNA
- Plasmid DNA
- Total cellular DNA
Quality Assessment
- DNA concentration
- Purity (A260/A280 ratio)
- Integrity assessed by agarose gel electrophoresis
Key Take-Home Message
DNA purification is the foundation of all molecular biology workflows.
Literature
Brown T. Gene Cloning and DNA Analysis - An Introduction 8ed (2020)
- Chapter 03 Purification of DNA from Living Cells