Summary: T3 - Polymerase Chain Reaction (PCR)
What is PCR?
PCR is a technique that amplifies a specific DNA sequence exponentially in vitro.
Core Components
- Template DNA
- Primers
- DNA polymerase
- dNTPs
- Thermal cycling
Applications
- Cloning
- Diagnostics
- Mutation detection
- Preparation for sequencing
Key Take-Home Message
PCR is powerful because it combines specificity with exponential amplification.
Literature
Brown T. Gene Cloning and DNA Analysis - An Introduction 8ed (2020)
- Chapter 09 The Polymerase Chain Reaction