Summary: T2 - DNA structure and manipulation
This class covers how purified DNA is cut, joined, modified, and analysed in vitro.
Core Techniques
- Restriction endonucleases
- DNA ligase
- DNA-modifying enzymes
- Agarose gel electrophoresis
Key Concepts
- Sticky ends vs blunt ends
- Restriction maps
- DNA fragment size determination
- DNA recovery from gels
Key Take-Home Message
DNA manipulation relies on enzyme specificity and molecular complementarity.
Literature
Brown T. Gene Cloning and DNA Analysis - An Introduction 8ed (2020)
- Chapter 04 Manipulation of Purified DNA