This protocol is quick but also dirty. There should be considerable amounts of phenol in the final preparation. Do not use for E. coli transformation, the phenol kills the E.coli.
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Glass beads (~150 µL)
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150 µL TE 10 X
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150 µL PCA (phenol : chloroform : isoamyl alcohol at proportion 25:24:1) (Plant lab)
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20 sec Fast Prep (or 5 min; max rpm; in the LGM disruptor)
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Centrifuge 10 min 14 000 rpm
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DNA is in the upper phase
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Dilute 10x and check in the NANODROP
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Use it as a template.
Reference for this protocol?