This protocol is quick but also dirty. There should be considerable amounts of phenol in the final preparation. Do not use for E. coli transformation, the phenol kills the E.coli.
- Glass beads (~150 µL)
- 150 µL TE 1 X
- 150 µL PCA (phenol : chloroform : isoamyl alcohol at proportion 25:24:1) (Plant lab)
- 20 sec Fast Prep (or 5 min; max rpm; in the LGM disruptor)
- Centrifuge 20 min at least 13 000 rpm
- DNA is in the upper phase
- Dilute 10x and check in the NANODROP
- Use it as a template.
Reference for this protocol?