ApE (A Plasmid Editor) is a freely available DNA Manipulation and visualization program. I works on macOS and Windows as well as on Linux.
It is not necessary to install ApE to use it, you can use it from a folder on your desktop.
Go here to get ApE, click on the link for your OS as indicated below.
Introduction to ApE
How to linearize a circular sequence at a specific location.
- Locate the cursor at the desired location where you want to cut the vector. Make sure sequence is circular.
- Select ApE Edit>“Linearize @ insert site” (see below).
The sequence is now linear beginning at the location of the cursor.
More info
